Table S1.
G-SB2KO mice have reduced serum free fatty acids after refeeding
| Serum, urine, and physical parameters | Control (n) | G-SB2KO (n) | P value |
| Serum | |||
| Insulin (ng/mL) | 0.54 ± 0.16 (3) | 0.55 ± 0.17 (5) | 0.97 |
| Leptin (ng/mL) | 5.9 ± 2.2 (3) | 3.7 ± 0.7 (5) | 0.28 |
| Cholesterol (mg/dL) | 122 ± 4 (3) | 112 ± 6 (5) | 0.29 |
| Triglycerides (mg/dL) | 45 ± 10 (3) | 46 ± 7 (5) | 0.96 |
| Free-fatty acids (mEq/L) | |||
| Fasting | 1.73 ± 0.10 (6) | 1.54 ± 0.08 (6) | 0.16 |
| Refed | 0.83 ± 0.08 (6) | 0.57 ± 0.06 (6) | 0.02 |
| Total T3 (ng/dL) | 57.7 ± 6.6 (6) | 52.9 ± 1.8 (6) | 0.50 |
| Total T4 (μg/dL) | 2.4 ± 0.4 (6) | 2.3 ± 0.1 (6) | 0.80 |
| Corticosterone (ng/mL) | 13.7 ± 1.9 (6) | 13.3 ± 2.0 (6) | 0.88 |
| Urine | |||
| Metanephrines (ng/mL) | 68.2 ± 10.2 (5) | 59.5 ± 5.8 (6) | 0.46 |
| Normetanephrines (ng/mL) | 1680 ± 220 (5) | 1630 ± 200 (6) | 0.87 |
| Physical | |||
| Body temperature (°C) | 36.5 ± 0.3 (8) | 36.4 ± 0.3 (10) | 0.94 |
| Fecal calorie content (calories/gram feces) | 4034 ± 24 (5) | 4043 ± 8 (6) | 0.72 |
| Blood pressure (mmHg) | |||
| Systolic | 107 ± 4 (5) | 106 ± 6 (6) | 1.00 |
| Diastolic | 93 ± 3 (5) | 84 ± 7 (6) | 0.31 |
| Pulse (beats per minute) | 602 ± 15 (5) | 652 ± 32 (6) | 0.22 |
Average ± SEM, n = the number of animals in each group. Insulin, leptin, cholesterol and triglycerides were measured from serum of fasting 3-mo-old male mice. Free-fatty acids were measured from the serum of 10-mo-old female mice after an overnight fast and then again after the same mice were refed. T3, T4, and corticosterone were measured on the same refed mice. Male mice were also used for fecal calorie content, urine metanephrines, and normetanephrines.