Table 1.
Summary of Tsc2 variants tested for their ability to rescue Tsc2-null mutant flies
| Construct | Description | No. of lines tested | No. of lines that rescue Tsc2-/- |
|---|---|---|---|
| Tsc2N1698K | GAP dead, disease-causing | 6 | 0 |
| Tsc2K1698A | GAP dead | 7 | 0 |
| Tsc2R884Q | Affecting Tsc1–Tsc2 complex, disease-causing | 5 | 0 |
| Tsc2R622W | Affecting Tsc1–Tsc2 complex, disease-causing | 5 | 0 |
| Tsc2AA | S924A/T1518A | 5 | 5 |
| Tsc2DE | S924D/T1518E | 12 | 12 |
| Tsc24A | T437A/S924A/T1054A/T1518A | 5 | 5 |
| Tsc2WT | Wild type Tsc2 | 3 | 3 |
All the constructs were tested for rescuing the null allele Tsc2192, as well as Tsc2193, another allele of Tsc2 that has an in-frame deletion (amino acids 472–527). Identical results were obtained using Tsc2192 and Tsc2193.