Figure 1. Peripheral blood γδ T-cell populations in donors analysed for γδ TCR repertoires.
(a) Major peripheral blood γδ T-cell populations identified by γδ TCR chain specific antibodies. Flow cytometry plots are representative of 20 donors. (b) Frequency of γδ T cells in CD3+ lymphocytes (left graph), abundance of Vδ2+ and Vδ2neg T cells in the γδ T cell compartment (Middle), and proportion of Vδ1+ T cells in the Vδ2neg γδ T-cell sub-population (Right). Graphs show the mean±s.e.m. from 20 donors. (c) Effect of donor CMV-seropositivity on CD8+, Vδ2+ and Vδ1+ cell percentages in total T cells. Graphs show the mean±s.e.m. from 10 CMV-seropositive and 10 CMV-seronegative donors. (d) Prevalence of a Vγ9 chain pairing in Vδ1+ T cells. Graphs show the mean±s.e.m. and flow cytometry plots are representative of 20 donors. Data analysed by student's t-test, *P=0.0228.