Fig. 5.
APP/PS1 mice exhibit hypothalamic insulin resistance and impaired BCAAs homeostasis. Western blot analysis of hypothalamic protein extracts from 5- to 6-month-old mice after the fasting-refeeding protocol (standard chow) and after high-fat feeding for 4 weeks (ITT plus insulin bolus) (A). Plasma BCAA concentration for young mice fed a standard chow diet (B) and after high-fat feeding (C) and old mice after high-fat feeding (D). Displayed are averages ± SEM. significance is defined as *P < .05. Wt n = 5 and APP/PS1 n = 4. Abbreviation: ITT, insulin tolerance test.