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. Author manuscript; available in PMC: 2017 Sep 15.
Published in final edited form as: Nature. 2017 Mar 15;543(7646):507–512. doi: 10.1038/nature21678

Figure 2. Seizures synergize with increased UBE3A to repress sociability and Cbln1.

Figure 2

a, LEFT: Seizure severity during 10 daily 30mg/kg PTZ doses in WT (n=25 males) and RIGHT, qRT-PCR for Cbln1 24h after last PTZ injection. b, Sociability (males), and c, USVs in male-female and treatment-matched female pairs. Seizure severity with 10 daily PTZ (30 mg/kg) and sociability 24h after last PTZ dose in d,f, WT (n=16) vs. p-Ube3amKO (n=17) and e,g, WT (n=18) and m-Ube3amKO (n=11). h, Baseline sociability, i, post-seizure sociability and j, seizure severity scores during the five day “subthreshold” PTZ paradigm in WT mice (n=8) and two independent cohorts of littermate Ube3a1x (untagged) mice (n=12 [saline], 14[PTZ]). k, Following seizures (above), qRT-PCR for Cbln1 and Ube3a mRNA in, p-Ube3amKO (n=5), m-Ube3amKO (9) and WT (7/group), and, Ube3a1x (untagged, n=8/group). Mean ± SEM plotted.