Fig. 6.
Derivatisation reactions for sterol and oxysterol analysis by LC-MS (A) Derivatisation with PTAD. (B) Derivatisation with GP reagent. (C) Left hand side, derivatisation with dimethylglycine; right hand side, derivatisation with nicotinic acid. Abbreviations: DMAP, 4-(dimethylamino)pyridine; DMG, N,N-dimethylglycine; EDC, 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide; DPC, N,N-diisopropylcarbodiimide.