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Qnr decreases gyrase binding to DNA. Gyrase binding to DNA was assessed by filter-binding assay and the elution fractions were analyzed by Southern blotting (Materials and Methods). Reaction mixtures contained 26 nM gyrase (lanes 2 to 5) and 1.9 μM Qnr-His6 (lanes 4 to 6). No protein was added to lane 1. Lane 3 was a duplicate of lane 2, and lane 5 was a duplicate of lane 4.
