Fig 1. Enzymatic activities involved in the glycolytic pathway.
Tg and WT astrocytes were treated or not with pantethine and then exposed or not to exogenous oligomeric Aβ (oAβ). Enzymatic activity and NADPH levels were determined on cell extracts. (A) Glucose-6-phosphate dehydrogenase (G6PD) activity. (B) Pyruvate kinase (PK) activity. (C) NADPH levels. Results are the mean values ± SD from three independent experiments (n = 3 per group). Enzyme activities are expressed as nmol/min/mg protein and NADPH levels are expressed as nmol/mg protein (*, significant difference between experimental groups; p<0.05). (D) Schematic representation of glucose metabolism enlightening the hexose monophosphate shunt (HMS) and PK (G6-P, glucose-6P; G-3P, glyceraldehyde-3P; PEP, phosphoenoylpyruvate; pyr, pyruvate).