Table 2.
Nuclear Receptors Target Discovery Using ChIP-Based Approaches
| Factor | Technique1 | Tissue/Cell Line | Bound Regions | Genomic Distribution |
|---|---|---|---|---|
| HNF4α | 2 | Human liver | 1555 | Promoters |
| HNF4α | 2 | Human pancreatic islets | 1423 | Promoters |
| ERα | 1 | MCF-7 | 12 | 8/12 <10 kb of TSS; 4/12 <2 kb of TSS |
| ERα | 1 | MCF-7 | 32 | n/a |
| ERα | 1 | MCF-7 | 94 | Intronic (45.7%); <10 kb upstream (12.8%); >50 kb upstream (17%) |
| ERα | 1 | U2OS | 173 | Intronic (38%); <10 kb upstream (24%); >10 kb upstream (38%) |
| ERα | 1 | Mouse uterus | n/a | n/a |
| ERα | 2 | MCF-7 | 153 | Promoters |
| ERα | 3 | MCF-7 | 57 | Mostly distal |
| ERα | 4 | MCF-7 | 3665 | Distal (96%) |
| ERα | 5 | MCF-7 | 1234 | Distal (95%) |
| ERα | 6 | MCF-7 | 578 | Promoters |
| ERα | 7 | MCF-7 | 47 | Promoters (79%) |
| ERα | 8 | MCF-7 | 92 | Promoters |
| ERα | 4 | Mouse liver | 5568 | >10 kb (60%) |
| GR | 9 | A549 | 8 | Promoters |
| GR | 10 | A549 | 73 | <5 kb from TSS of nearest responsive gene (31%) |
| AR | 3 | LNCaP | 90 | <500 kb from TSS of nearest responsive gene (38%) |
| AR | 11 | HPr-1AR | 524 | <10 kb from TSS of nearest responsive gene (33%) |
| AR | 2 | LNCaP | 1532 | Promoters |
| ERRα and ERRγ | 2 | Mouse heart | 736 | Promoters |
| ERRα | 2 | Mouse bone-derived macrophages | 215 | Promoters |
| VDR | 12 | ST2 | 3 (VDR) 5 (RANKL) | Intronic (VDR) >15 kb upstream TSS (RANKL) |