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. 2005 Jan;25(2):661–670. doi: 10.1128/MCB.25.2.661-670.2005

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Copyright © 2005, American Society for Microbiology

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FIG. 3. — Phosphorylation of ATM, p53, and Chk2 in NBS1^+/+ and NBS1^m/m cells after IR. Phosphorylation of ATM at Ser1981 in NBS1^+/+ and NBS1^m/m MEFs (A) and thymocytes (B) 1 h after treatment with 1 Gy of IR or in NBS1^+/+ and NBS1^m/m thymocytes 10 min after 1 Gy of IR (C). The treatment and genotypes are indicated at the top. Phosphorylated ATM and total ATM are indicated on the right. Phosphorylation of p53 in NBS1^+/+ and NBS1^m/m MEFs 1 h after various dosages of IR (D) and thymocytes 1 h after treatment with 1 Gy of IR (E). The treatment and genotypes are indicated at the top. Phosphorylated p53 and total p53 are indicated on the right. Phosphorylation of H2AX (F) and Chk2 (G) in ATM^−/−, NBS1^+/+, and NBS1^m/m thymocytes 1 h after treatment with 1 Gy of IR. The genotypes and treatment are indicated at the top. Phosphorylated H2AX and Chk2 are indicated on the right.