Figure 3.
Increased IL-13 production from cells lacking lineage markers (lineageneg) CD127+ innate lymphoid cells in chronic rhinosinusitis (CRS) with nasal polyps (CRSwNP) in response to IL-33 stimulation. IL-13 production from CD45+ cells isolated from inflamed sinonasal mucosa of (A) patients with CRSwNP (n = 10), or (B) patients with CRS without nasal polyps (CRSsNP) (n = 3) stimulated with recombinant IL-2, IL-33, or both was determined by ELISA. (C) IL-13 production from CD45+ cells from CRSwNP (n = 10) and CRSsNP (n = 3) stimulated with recombinant IL-2 and IL-33 was determined by ELISA. Each point represents the results for an individual patient sample, and the horizontal line represents the mean. Results were analyzed by one-way analysis of variance with Tukey posttest (A, B) or Mann-Whitney test (C). P < 0.05 was considered significant. **P < 0.01 and ***P < 0.001.