Figure 5.
The NTD of TTP and BRF-1 can activate mRNA decay when tethered via the MS2 coat protein. (A) Northern blots showing decay rates of a β-globin mRNA with six MS2 coat protein-binding sites in the 3′UTR (β-6bs), in the presence of exogenous MS2, TTP, MS2-TTP, and MS2-BRF-1 proteins indicated on the left. Calculated β-6bs mRNA half-lives and the average fold change from the assay with the MS2 protein alone are given on the right (four experiments). (B) Northern blots showing decay rates of β-6bs mRNA, in the presence of tethered NTDs of TTP and BRF-1 proteins indicated on the left. Calculated β-6bs mRNA half-lives and average fold change are given on the right (three and four experiments).