FIG. 7.
SOCS-3 induced by resistin associates with the IR. 3T3-L1 adipocytes were treated with resistin (30 ng/ml) or TNF-α (30 ng/ml) for the indicated times. (a) Treatment with TNF-α, but not resistin, reduces IRS-1 protein levels. Actin immunoblot analysis was used as a loading control. (b) Resistin stimulates IR association of SOCS-3. Cell extracts were immunoblotted (IB) for IR and SOCS-3 following immunoprecipitation (IP) of IR. (c) Time course of resistin enhancement of IR association with SOCS-3.