Table 2. Minimal kinetic parameters for λQ33Y in aqueous buffer for 260 M protein.
T, K | Probe | A (A′ = 1 - A) | k, μs-1 | k′, μs-1 |
---|---|---|---|---|
336 | Fl. | 0.67 (3) | 0.036 (5) | 0.5 (2) |
IR | 1 | 0.12 (1) | — | |
341 | Fl. | 1 | 0.069 (3) | — |
IR | 1 | 0.070 (5) | — | |
348 | Fl. | 1 | 0.37 (13) | — |
IR | 1 | 0.45 (8) | — |
Fl., fluorescence.
*The uncertainties are 1σ precisions of the fit.