Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jul 11;8:16026. doi: 10.1038/ncomms16026

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Methylation levels (y axis) at Target 1 (on-target) between HOXA5 and HOXA6 loci along the length of the CGI (x axis). Red bars indicate the locations of sgRNA-binding sites and PAM is located on the right side of sgRNA-binding site. The guide RNAs included in the experiment depicted are indicated in each panel, with sg1-3 including all three HOXA5 Target1 guides. (b) Western blotting for protein expression levels of various dCas9-MQ1 mutant fusion proteins in HEK293T cells. Anti-FLAG antibody was utilized and β-actin was selected as internal control. (c) DNA methylation analysis as above when guides targeting the HOXA4 or EYA4 loci are co-transfected with dCas9-MQ1^Q147L. Red bars indicate the locations of HOXA4 and EYA4 sgRNA-binding sites. The methylation ratio of CpG sites was calculated based on three biological replicates. Error bars represent mean±s.e.m. of biological triplicates.