Fig. 6.
Comparison between HPLC and EPR spin-trapping detection of 
in BAECs treated with menadione. (A Upper) BAECs were treated with menadione (10 μM) for 1 h, then washed with DPBS and incubated with HE (10 μM) for 20 min. (Lower) BAECs were preincubated with BMPO (25 mM) and then treated with menadione (10 μM) and HE (10 μM). HE-derived oxidation products were obtained in cell lysates as described earlier. (B) Experimental conditions were the same as in A except that the HE-derived products were analyzed in the cell culture medium. (C Upper) BAECs were treated with menadione (10 μM) and BMPO (25 mM) for 1 h. EPR spectra of cell lysates were recorded. (Lower) Experimental conditions were the same as in Upper except for the presence of SOD (100 units/ml). (D) Experimental conditions were the same as in C except that EPR spectra of media were analyzed.