Skip to main content
NCBI home page
The EMBO Journal logoLink to The EMBO Journal
. 1984 Jan;3(1):23–27. doi: 10.1002/j.1460-2075.1984.tb01756.x

Cloning and sequencing of a full length cDNA coding for a human apoferritin H chain: evidence for a multigene family.

F Costanzo, C Santoro, V Colantuoni, G Bensi, G Raugei, V Romano, R Cortese
PMCID: PMC557292  PMID: 6323167

Abstract

We have cloned a segment of cDNA from human liver coding for an apoferritin subunit, probably an H chain. Sequence comparison with the available protein sequence shows that our clone corresponds to a ferritin subunit present as a minor species in human spleen and placenta, but as major species in HeLa cells. Northern blot analysis shows the existence of only one band of similar size in human liver, HeLa cells, Daudi lymphoma and Hep3B hepatoma cell lines. In contrast, Southern blot analysis provides evidence for a multigene family.

Full text

PDF
23

Images in this article

25Fig. 4.
on p.25
26Fig. 5.
on p.26
26Fig. 6.
on p.26

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aisen P., Listowsky I. Iron transport and storage proteins. Annu Rev Biochem. 1980;49:357–393. doi: 10.1146/annurev.bi.49.070180.002041. [DOI] [PubMed] [Google Scholar]
  2. Arosio P., Adelman T. G., Drysdale J. W. On ferritin heterogeneity. Further evidence for heteropolymers. J Biol Chem. 1978 Jun 25;253(12):4451–4458. [PubMed] [Google Scholar]
  3. Brown A. J., Leibold E. A., Munro H. N. Isolation of cDNA clones for the light subunit of rat liver ferritin: evidence that the light subunit is encoded by a multigene family. Proc Natl Acad Sci U S A. 1983 Mar;80(5):1265–1269. doi: 10.1073/pnas.80.5.1265. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cortese R., Harland R., Melton D. Transcription of tRNA genes in vivo: single-stranded compared to double-stranded templates. Proc Natl Acad Sci U S A. 1980 Jul;77(7):4147–4151. doi: 10.1073/pnas.77.7.4147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cortese R., Melton D., Tranquilla T., Smith J. D. Cloning of nematode tRNA genes and their expression in the frog oocyte. Nucleic Acids Res. 1978 Dec;5(12):4593–4611. [PMC free article] [PubMed] [Google Scholar]
  6. Costanzo F., Castagnoli L., Dente L., Arcari P., Smith M., Costanzo P., Raugei G., Izzo P., Pietropaolo T. C., Bougueleret L. Cloning of several cDNA segments coding for human liver proteins. EMBO J. 1983;2(1):57–61. doi: 10.1002/j.1460-2075.1983.tb01380.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gronenborn B., Messing J. Methylation of single-stranded DNA in vitro introduces new restriction endonuclease cleavage sites. Nature. 1978 Mar 23;272(5651):375–377. doi: 10.1038/272375a0. [DOI] [PubMed] [Google Scholar]
  8. Hazard J. T., Drysdale J. W. Ferritinaemia in cancer. Nature. 1977 Feb 24;265(5596):755–756. doi: 10.1038/265755a0. [DOI] [PubMed] [Google Scholar]
  9. Hu N., Messing J. The making of strand-specific M13 probes. Gene. 1982 Mar;17(3):271–277. doi: 10.1016/0378-1119(82)90143-3. [DOI] [PubMed] [Google Scholar]
  10. Morgan M. A., Hoffbrand A. V., Laulicht M., Luck W., Knowles S. Serum ferritin concentration in Gaucher's disease. Br Med J (Clin Res Ed) 1983 Jun 11;286(6381):1864–1864. doi: 10.1136/bmj.286.6381.1864. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Proudfoot N. J., Brownlee G. G. 3' non-coding region sequences in eukaryotic messenger RNA. Nature. 1976 Sep 16;263(5574):211–214. doi: 10.1038/263211a0. [DOI] [PubMed] [Google Scholar]
  12. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  14. Vieira J., Messing J. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene. 1982 Oct;19(3):259–268. doi: 10.1016/0378-1119(82)90015-4. [DOI] [PubMed] [Google Scholar]
  15. Woods D. E., Markham A. F., Ricker A. T., Goldberger G., Colten H. R. Isolation of cDNA clones for the human complement protein factor B, a class III major histocompatibility complex gene product. Proc Natl Acad Sci U S A. 1982 Sep;79(18):5661–5665. doi: 10.1073/pnas.79.18.5661. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The EMBO Journal are provided here courtesy of Nature Publishing Group

RESOURCES