Figure 3.
Minus and Plus mutations of core UBF prevent the formation of the 360° DNA loop characteristic of the wild-type enhancesome. (A) Dimers of Minus and Plus core UBF mutants complexed with DNA. (B) Typical enhancesome complexes containing dimers of wild-type core UBF complexed to DNA. (A) and (B) show superimposed phosphorus (DNA in red) and total mass (grey) ESI images of wild-type and core UBF mutants bound to the 1.1 kb Xenopus ribosomal enhancer DNA. The calculated protein mass for each complex is given.