Fig. 6.
NSA blocks NTD polymer formation in a cysteine 86-dependent manner to block necroptosis. (A) Recombinant NTD or NTD-C86S (1 μM) was incubated at 37 °C with or without NSA, and 50 ng protein from each treatment was loaded for SDD-AGE. (B and C) NTD-DmrB or NTD-C86S-DmrB cells were treated as indicated. Cell lysates were subjected to nonreducing or reducing SDS/PAGE (B) or SDD-AGE (C). (D) NTD-DmrB or NTD-C86S-DmrB cells were subjected to the indicated treatments, and cell survival was determined by CellTiter-Glo assay. Data are presented as mean ± SD. IB, immunoblot.