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MLKL polymers are high molecular weight complexes. DMSO- or T/S/Z-treated cell lysates were subjected to Superdex 200 gel filtration fractionation. Fractions eluted at 7.5 mL, 8.5 mL, and 14.5 mL were analyzed by SDD-AGE and SDS/PAGE and detected with Flag antibody. IB, immunoblot.