Figure 2.
Runx3 is essential for the differentiation and long-term maintenance of CD8+ Trm cells.
a, Ratio of Runx3- or control-shRNAmir transduced P14 cells in indicated tissues on day 23 or 26 of LCMV infection. b, Ratio of transduced transferred cells in indicated tissues on day 32 of enteric LM-GP33–41 infection. c, Ratio of transferred Runx3fl/flErt2-Cre+YFP (Runx3fl/fl) and Runx3+/+Ert2-Cre+YFP (Runx3+/+) P14 cells in indicated tissues on days 6/7 and 15/16 of LCMV infection (top) or enteric LM-GP33–41 infection (bottom). d, Ratio Runx3fl/fl and Runx3+/+ P14 cells on day 15 of LCMV infection. e, Ratio of Runx3fl/fl and Runx3+/+ P14 cells on day 35 of infection. Graphs indicate mean ± s.e.m of n=5 (a), n=3 (b), n=3 (day 7) and n= 6 (day 15/16) (c), n=5 (d), and n=5 (vehicle) and n=3 (tamoxifen) (e). All data are from one representative experiment of 2 independent experiments except a is pooled from 2 independent experiments; *P<0.05, **P<0.01, ***P<0.005. Symbols represent an individual mouse (a–e).