Extended Data Figure 5. Runx3 is required for Trm formation in diverse non-lymphoid tissues.
a, Schematic of experimental design. b, Representative flow cytometry plots (left) and quantification (right) of the ratio Runx3fl/fl and Runx3+/+ P14 cells (gated on YFP-Cre+ cells) in lymphoid and non-lymphoid compartments on days 15/16 of LCMV infection (same data as in Fig. 2d but including SG and kidney populations). c, Schematic for experimental design. d, Representative flow cytometry plots (left) and quantification (right) of the ratio of transduced cells in the skin relative to the spleen for Con shRNAmir or Runx3 shRNAmir P14 cells on day 12 of an intradermal (i.d.) LCMV infection. e, Frequency of CD69+ and CD103+ cells. f, Schematic for experimental design. g, Representative flow cytometry plots (left) and quantification (right) of the ratio of transduced cells in the lung parenchyma relative to the spleen for Con shRNAmir or Runx3 shRNAmir P14 cells on day 12 of an intratracheal (i.t.) LCMV infection. h, Frequency of CD69+ and CD103+ cells. Graphs indicate mean ± s.e.m and representative of two independent experiments with n=6 (b), or data pooled from two individual experiments with n=6 per group (c–h), *P<0.05, **P<0.01, ***P<0.005. Symbols represent an individual mouse (b,d,e,g,h).