Figure 1.
NKT Cell-Deficient Mice Exhibit Impaired Antiviral B Cell Responses
(A and B) Flow cytometry analysis of mediastinal lymph nodes (LN) from control (black), wild-type (blue), or CD1d−/− (red) mice on day 9 of influenza infection. Contour plots display the percentage of (A) germinal center and (B) TfH cells.
(C) Confocal microscopy images of mediastinal lymph node sections from mice treated as in (A) and (B), stained with antibodies to IgD (green) and GL7 (magenta). Scale bar, 450 μm.
(D) Quantification of the number and follicular area occupied by germinal centers in confocal images shown in (C) by ImageJ.
(E) ELISPOT analysis of IgG1 influenza-specific ASCs in mediastinal lymph nodes from wild-type and CD1d−/− mice treated as described in (A) and (B).
(F and G) Flow cytometry analysis of mediastinal lymph nodes of from control (black), wild-type (blue), or CD1d−/− (red) mice on day 9 of vaccinia virus infection. Contour plots display the percentage of (F) germinal center and (G) TfH cells.
Data show one representative result from three experiments, where each dot represents one mouse. Data represent mean ± SEM; two-tailed paired Student’s t test, ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗∗p < 0.0001.