Figure S1.
NKT Cell-Deficient Mice Display Early Impairment in B Cell Immunity, Related to Figure 1
(A–D) Flow cytometry analysis of mediastinal lymph node cells from wild type or CD1d−/− mice that were intranasally infected with 200 PFU of influenza virus for (A-B) 7 or (C-D) 21 days. Representative contour plots display the percentage of (A-C) B220+ cells bearing biomarkers of germinal cell activity, Fas+GL7+, and (B–D) CD4+ T cells bearing biomarkers of TfH cells, CXCR5+PD-1+. Dot plots show quantification of (A-C) germinal center and (B-D) TfH cells.
(E and F) Flow cytometry analysis of mediastinal lymph nodes harvested at day 10 from wild type mice that were intranasally challenged with (E) 10 μg of HA trimmer or (F) PBS in the presence or absence of poly I:C or α-GalCer. Representative contour plots display the percentage of HA-specific B220+ cells differentiated into Fas+GL7+ germinal center cells.
(G and I) ELISPOT analysis of IgG1 HA-specific ASCs in mediastinal lymph nodes from wild type and CD1d−/− mice treated as in figure S1E.
(H and J) Flow cytometry analysis of HA-specific germinal center cells in mediastinal lymph nodes from wild type and CD1d−/− mice treated as in figure S1E.
In all quantification charts, each dot represents one mouse. Data are representative from two experiments. Statistical analysis two-tailed Student’s t test; ∗p < 0.05.