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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Biol Blood Marrow Transplant. 2017 Oct 9;24(2):242–251. doi: 10.1016/j.bbmt.2017.10.003

Figure 5. MAIT cell reconstitution after HCT correlates with the abundance of distinct GI microbial species.

Figure 5

(A) TRBV nucleotide sequence diversity as measured by the productive entropy in MAIT cells from healthy donors (n=3), PBSC grafts (n=5) and paired HCT recipients (n=5) at the indicated days after HCT. Lines connect samples from each donor-recipient pair. (B) Percentage of TRBV nucleotide sequences from MAIT cells at day 90 and 1 year after HCT that were also identified at day 30 (left, middle), and of sequences identified at 1 year that were also present at day 90 (right, n=5). (C) Percentage of total unique TRBV sequences shared between the PBSC graft, and the recipient on day 30 or 90 after HCT. Data from two representative HCT recipients are shown. Each color represents a unique TRBV nucleotide sequence. (D) Principal components analysis (coordinates 1 and 2) of the composition of the GI microbiota. Each colored line connects samples from a single PBSC recipient at distinct times after HCT (n=15). Open circles indicate samples collected before HCT and closed circles are samples collected after HCT. A short distance between points indicates similarity between the composition of the microbiota at each timepoint. (E) Correlation between blood MAIT cell counts and the relative abundance in stool of Blautia spp. and Bifidobacterium longum (n=54 paired blood and stool samples). Log-log linear regression Pearson correlation coefficient r and p values are shown.