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. 2018 Apr 9;128(5):1919–1936. doi: 10.1172/JCI91646

Figure 9. Analogous alterations in the microbiome in the absence of MAIT cells and IL-17A.

Figure 9

(A) Separately housed B6.WT and B6.IL-17A–/– mice were transplanted with 25 × 106 G-CSF–mobilized BALB/c splenocytes, and clinical scores and survival were monitored. Data pooled from 2 independent experiments (n = 10 per group; TCD group, n = 3; B6.IL-17A–/– TCD group, n = 5). Survival data analyzed with a log-rank test. P < 0.0001, B6.WT versus B6.IL-17A–/–. (B) Fecal samples collected from naive B6.WT and B6.IL-17A–/– mice were profiled by 16S rRNA-seq. Principal component analysis of B6.WT, B6.IL-17A–/–, and B6.MR1–/– (Figure 7A data set) data demonstrates distinct OTU profiles. Each dot represents an individual mouse, with symbols and colors corresponding to the genetic background and experiment. Data from 4 experiments are shown. Experiment 1, WT versus MR1–/–, n = 6 mice per group; experiments 2 and 3, WT versus MR1–/–, n = 4-5 mice per group; experiment 4, WT versus IL-17A–/–, n = 8-10 mice per group. (C) Heatmap showing significantly different OTUs between naive B6.IL-17A–/– and B6.MR1–/– mice in comparison with B6.WT mice from the same samples as used in B. Details contained in Supplemental Table 3. 16S rRNA sequencing data analyzed using DESeq2.