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| Localization | Concentration (µM) (± SD) |
N cells |
|---|---|---|
| Cytosol | 1.6 (± 1.4) | 84 |
| Nucleus | 4.0 (± 3.3) | 51 |
| Mitochondria | 4.7 (± 1.3) | 49 |
Concentrations were determined in U2OS cells using a confocal fluorescence microscope by singly labeled the sensor construct with SiR-Halo and CP-TMR-SMX and comparing with the purified sensor calibration curves in buffer using identical microscope settings.
