Skip to main content
. 2018 Mar 9;96(6):666–674. doi: 10.1111/imcb.12020

Figure 3.

Figure 3

Upregulation of granzyme B in MAIT cells and “Non‐MAIT” CD8+ T cells. (a) MAIT cells were assayed for the intracellular expression of granzyme B in the presence or absence of indicated stimuli (cytokines IL‐12 and IL‐18 (50 ng mL −1) ± anti‐CD3/CD28 stimulation) in the presence or absence of 2‐deoxyglucose (2 mmol L−1). Significance was determined using a paired, parametric two‐tailed t‐test. Significance levels: * indicates P < 0.05, **P < 0.01, ***P < 0.001. Error bars show mean ± s.d. Cumulative data are shown for n = 3 healthy donors (representative of two independent experiments). (b) “Non‐MAIT” CD8+ T cells were assayed for the intracellular expression of granzyme B in the presence or absence of indicated stimuli (cytokines IL‐12 and IL‐18 (50 ng mL −1) ± anti‐CD3/CD28 stimulation) in the presence or absence of 2‐deoxyglucose (2 mM). Significance was determined using a paired, parametric two‐tailed t‐test. Significance levels: * indicates P < 0.05, **P < 0.01, ***P < 0.001. Error bars show mean ± s.d. Data are shown for n = 3 healthy donors (representative of two independent experiments). (c) MAIT cells were assayed for the expression of CD69 in the presence or absence of indicated stimuli (cytokines IL‐12 and IL‐18 (50 ng mL −1) ± anti‐CD3/CD28 stimulation) in the presence or absence of 2‐deoxyglucose (2 mmol L−1). Significance was determined using paired, parametric two‐tailed t‐test. Significance levels: * indicates P < 0.05, **P < 0.01, ***P < 0.001. Error bars show mean ± s.d. Data are shown for n = 3 healthy donors (representative of two independent experiments). (d) “Non‐MAIT” CD8+ T cells were assayed for the expression of CD69 in the presence or absence of indicated stimuli (cytokines IL‐12 and IL‐18 (50 ng mL −1) ± anti‐CD3/CD28 stimulation) in the presence or absence of 2‐deoxyglucose (2 mmol L−1). Significance was determined using a paired, parametric two‐tailed t‐test. Significance levels: * indicates P < 0.05, **P < 0.01, ***P < 0.001. Error bars show mean ± s.d. Data are shown for n = 3 healthy donors (representative of two independent experiments).