Extended Data Figure 9. Treatment with L. murinus or L. reuteri ameliorates salt-sensitive hypertension.
(a) Mean diastolic pressures over time in response to HSD and HSD with concomitant L. murinus treatment in n=7 FVB/N mice. Scale bar indicates 24 hours. Horizontal line indicates the mean across all values of the respective phase. (b, c) Mean systolic (b) and diastolic (c) blood pressures in these mice (n=7) fed a HSD (black curve) and HSD with concomitant L. murinus treatment at circadian scale. Arrows indicate the time of L. murinus gavage. (d, e) Boxplots (median, IQR, whiskers 1.5*IQR) show systolic (d) and diastolic (e) blood pressures recorded continuously in FVB/N mice fed a HSD and a HSD with concomitant L. reuteri treatment. These mice (n=9) were fed a HSD for 10 days prior to concomitant L. reuteri treatment for another 7 days. ***p<0.001 vs. HSD using linear mixed model. (f, g) Boxplots (median, IQR, whiskers 1.5*IQR) show systolic (f) and diastolic (g) blood pressures in mice (n=5) fed a HSD and a HSD with concomitant Escherichia coli Nissle 1917 (E. coli) treatment for 3 days, respectively. Statistics using linear mixed model. (h-j) Quantification of CD4+IL-17A-IFN-γ+ lymphocytes in siLPL (h, n=5 for NSD, n=7 for HSD, n=6 for HSD+L. murinus) and cLPL and spleen, respectively (i-j, n=5 for NSD, n=6 for HSD, n=6 for HSD+L. murinus). All bars show mean±s.e.m, circles represent independent mice. *p<0.05 using Kruskal-Wallis and Dunn’s post-hoc test for (h), one-way ANOVA for (i-j).