Figure 8. High-resolution refinement: apo-ferritin (A) FSC curves between independently refined half-maps at different stages of the processing.
(B) FSC curves between PDB-5N27 (Ferraro et al., 2017) and the cryo-EM map at different stages of the processing. (C) Representative density features at different stages of the processing.
Figure 8—figure supplement 1. The B-factor plot that was generated automatically by the bfactor_plot.py script.
The script performed ten 3D refinement and post-processing jobs with subsets of increasing sizes for the high-resolution apo-ferritin data set. Fitting a straight line through the inverse of the resolution squared of each refinement against the natural logarithm of the number of particles in the corresponding subset yielded an estimated B-factor of 66 Å2.