Table 2.
Quantification Methods for Soyasapogenol A and B.
| Compound | MW | Formula | Analysis Method | Solvent System and Program | Specification |
|---|---|---|---|---|---|
| Soyasapogenol A | 474 | C30H50O4 | TLC [33] Silica gel 60G | Light petroleum (b.p. 60-80 °C), ethyl acetate (4:3) (v/v) | Visualization 10% sulfuric acid in ethanol and viewing under UV |
| TLC [34] | Dichloromethane and methanol (9:1) (v/v) | Spraying with a saturated solution of potassium dichromate in sulfuric acid | |||
| Normal HPLC [33] | A: Light petroleum (b.p. 60-80°C); B: Ethanol, 0-7.5min, 0-7.5% B; 7.5-15 min, 7.5% B isocratic; 15-20 min, 7.5-20% B |
Silica Column (250mm × 4.6mm) Flow-rate 1.5 mL/min |
|||
| Soyasapogenol B | 458 | C30H50O3 | Revised HPLC [12] | Acetonitrile: 1-propanol: water: 0.1% acetic acid (80:6:13.9:0.1) (v/v) Isocratic |
ODS C18 column (250mm × 4.6mm) Flow-rate 0.9mL/minELSD detection |
| Revised HPLC [35] | A: Acetonitrile: 1-propanol: water: acetic acid (80:6:13:0.1) (v/v); B: 100% Acetonitrile 0-15 min 100% A isocratic; 15-17 min 0-100% B; 17-19 min 100% B; 19-22 min back to 100% A |
RP-C18-AB column (250 × 4.6 mm) Flow-rate 0.9 mL/min |