Fig. 5.
The XMG1.2 antibody neutralizes the activity of IFN-γ in vitro. As reported previously (Yong et al., 1992), IFN-γ inhibits the proliferation of neonatal mouse astrocytes in a dose-dependent manner. Here, a single concentration of 1000 U/ml recombinant murine IFN-γ was applied to neonatal mouse astrocytes for 48 hr, and 3H-thymidine was determined. The inhibitory effect of IFN-γ on the proliferation of neonatal mouse astrocytes was reversed by 0.5–10.0 μg/ml of XMG antibody. The hatched columns to the right demonstrate that the XMG antibody by itself did not affect cell proliferation. Each bar is the mean ± SEM of four coverslips of cells. p < 0.05 compared with IFN-γ alone (one-way ANOVA with Duncan’s multiple comparisons, with p set at 0.05).