Fig. 10.
Aβ domains and interactions with microglia.A, Phase photomicrograph of rat microglial cell adhering to Sepharose bead coupled to human Aβ1-42 peptides. B, Fluorescence photomicrograph of the same bead showing adherent cell labeled by the fluorescent microglial marker DiI-ac-LDL. Scale bar, 20 μm. C, Rat microglial adherence to Sepharose-coupled beads after 6 hr. Plaque proteins derived from neuritic/core plaques provided an anchoring site for microglia, as did Aβ1-42. Importantly, Aβ1-28 also promoted bead binding, whereas Aβ17-43 did not. Controls included beads coupled to glycine (Control-glycine) and to bovine serum albumin (Control-BSA). Data shown are expressed as the numbers of adhering cells per 100 randomly selected beads ± SE after 6 hr incubation at 37°C.