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. 1996 Oct 1;16(19):6021–6037. doi: 10.1523/JNEUROSCI.16-19-06021.1996

Fig. 6.

Fig. 6.

Aβ activates microglia after coupling to microspheres. Fluorescently labeled microspheres were covalently coupled to Aβ1-42 and placed in hippocampal cultures containing rat microglia (500 cells/mm2). After 72 hr, Aβ1-42 spheres (A) were localized specifically within DiI-ac-LDL(+) microglia (B; colocalization noted byarrows). In contrast, Aβ17-43 microspheres (C) showed no consistent association with microglia (D). Scale bar, 20 μm. E, Comparison of capacity of Aβ in solution or coupled to microspheres (Bead-Bound) to elicit neurotoxic microglia (250,000 microspheres/culture; 100,000 microglia/culture; 72 hr incubation). Neuronal loss was similar whether Aβ peptides were in solution or bound to beads, indicating that fibril formation or other changes in tertiary structure were not necessary to stimulate neurotoxic microglia.