Table 1.
LRRK2 interactors
| Identified proteins | Accession number | Molecular weight | n peptides | Domain | Function |
|---|---|---|---|---|---|
| α-Actin-2 | P62737 | 42 kDa | 10 | WD40 | Component of cytoskeleton |
| AP-1 complex subunit β-1 | O35643 | 104 kDa | 8 | WD40 | Required for vesicle sorting during endocytosis |
| AP-2 complex subunit α-1 | P17426 | 108 kDa | 5 | WD40 | AP-2 complex is a heterotetramer; it mediates the recruitment of clathrin to membranes and plays a role in the recycling of synaptic vesicle membranes from the presynaptic surface |
| AP-2 complex subunit α-2 | P17427 | 104 kDa | 7 | ANK | |
| AP-2 complex subunit β-1 | Q9DBG3 | 105 kDa | 5 | WD40 | |
| Calcium-dependent secretion activator 1 | Q80TJ1 | 153 kDa | 3 | WD40 | Calcium-binding protein involved in the exocytosis of synaptic vesicles |
| Clathrin coat assembly protein AP180 | Q61548 | 92 kDa | 6 | WD40 | Components of the adapter complex that links clathrin to coated vesicles |
| Clathrin heavy chain 1 | Q68FD5 | 192 kDa | 52 | WD40 | Main coat of coated pits and vesicles |
| Dynamin-1 | P39053 | 97 kDa | 27 | WD40 | Microtubule-associated protein involved in endocytosis of vesicles |
| Synapsin-1 | O88935 | 74 kDa | 4 | WD40 | Neuronal phosphoprotein that coats synaptic vesicles |
| Synaptic vesicle glycoprotein 2A | Q9JIS5 | 83 kDa | 5 | WD40 | Regulates vesicle fusion by maintaining the readily releasable pool |
| Syntaxin 1B | P61264 | 33 kDa | 5 | WD40 | Involved in docking of synaptic vesicles at presynaptic active zones |
| Vesicle-fusing ATPase | P46460 | 83 kDa | 21 | WD40 | Required for vesicle-mediated transport |
A domain-based GST pull-down approach was performed to explore LRRK2 interactome. GST fusion proteins covering full-length LRRK2 and mimicking its functional domain (GST–N-term, GST–ANK, GST–LRR, GST–R-C-K, and GST–WD40) was used to retain interactors from adult mouse brain lysate. The nature of the pulled proteins including putative interactors was identified by LC-MS/MS. Peptide identifications were accepted if they could be established at greater than 95%, whereas protein identifications were accepted if they could be established at greater than 99% probability and contained at least two identified unique peptides. Only hits confirmed by more than two independent experiments and absent in the GST control sample were taken into consideration. The table reports protein name, UniProtKB/Swiss-Prot accession number, protein molecular weight, number of unique peptides identifying the indicated protein, GST fusion domain bound, and protein putative function as annotated in UniProt database. Supplemental Table 1 reports additional peptides information (available at www.jneurosci.org as supplemental material).