Figure 4.
Expression of TrkB and p75NTR in the adult RMS. A, In situ hybridization for TrkB in the adult SVZ–OB pathway. Note the intense labeling in the adult RMS. Scale bar, 100 μm. LV, Lateral ventricle; Str, striatum. B, High-magnification images of the boxed areas in A. Scale bar, 10 μm. C, RT-PCR analysis of FACS-purified astrocytes (GFAP) and neuroblasts (PSA-NCAM). Note the high level of expression of p75NTR in neuroblasts and TrkB receptors in astrocytes. D, Immunostaining for TrkB (green), Dcx (red), and GFAP (blue) in the adult mouse RMS. Scale bar, 100 μm. E, F, Immunostaining for TrkB (green) and GFAP (red) in the adult mouse RMS. F, High-magnification images of the boxed area in E. Scale bars: E, 100 μm; F, 10 μm. G, H, Micrographs showing that most, if not all, of TrkB (green) immunopositivity is not localized in Dcx-immunopositive cells (red). H, High-magnification images of the boxed area in G. Scale bars: G, 100 μm; H, 10 μm. I, J, Micrographs displaying expression of p75NTR (green) by migrating neuroblasts (Dcx, red). J, High-magnification images of the boxed area in (I). Scale bar, 10 μm.