Figure 4.
Validation of gene expression data by in situ hybridizations. A–C, Coronal brain sections showing ISH for genes identified by array data as having specific neuronal expression: A, Nov; B, Tmem130; C, Brunol4. D–L, Higher-magnification images corresponding to outlined region in A showing hippocampus, corpus callosum (cc), and the overlying cortex. D–F, Genes identified by array data as having specific neuronal expression, displaying expression in the hippocampus and cortex: D, Nov; E, Tmem130; F, Brunol4. G–I, Genes identified by array data as having astrocyte- enriched expression, showing fibrous, positive cells throughout the white and gray matter: G, Ntsr2; H, Aldh1L1; I, Acsbg1. J–L, ISH for genes identified by array data as having specific OL expression, showing white matter expression in the corpus callosum and the occasional positive cell in the overlying cortex: J, Fa2h; K, Tmem125/6330530A05Rik; L, Gpr62. All ISH performed on P17 mouse brains. Scale bars: A–C, 2 mm; D–L, 200 μm.