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. 2008 Jan 2;28(1):264–278. doi: 10.1523/JNEUROSCI.4178-07.2008

Figure 4.

Figure 4.

Validation of gene expression data by in situ hybridizations. A–C, Coronal brain sections showing ISH for genes identified by array data as having specific neuronal expression: A, Nov; B, Tmem130; C, Brunol4. D–L, Higher-magnification images corresponding to outlined region in A showing hippocampus, corpus callosum (cc), and the overlying cortex. D–F, Genes identified by array data as having specific neuronal expression, displaying expression in the hippocampus and cortex: D, Nov; E, Tmem130; F, Brunol4. G–I, Genes identified by array data as having astrocyte- enriched expression, showing fibrous, positive cells throughout the white and gray matter: G, Ntsr2; H, Aldh1L1; I, Acsbg1. J–L, ISH for genes identified by array data as having specific OL expression, showing white matter expression in the corpus callosum and the occasional positive cell in the overlying cortex: J, Fa2h; K, Tmem125/6330530A05Rik; L, Gpr62. All ISH performed on P17 mouse brains. Scale bars: A–C, 2 mm; D–L, 200 μm.