Figure 10.
Altered synaptic responses in Mint-deficient neurons. A, B, Sample traces showing miniature excitatory and inhibitory recordings of cremut and crewt at 13–15 DIV. Bar graphs of the quantification revealed a significant decrease in miniature frequency but not amplitude in neurons transfected with crewt that abolished all three Mint proteins. C, D, Sample traces showing sucrose-evoked excitatory and inhibitory recordings of cremut and crewt at 13–15 DIV. Bar graphs showed a significant decrease in sucrose-evoked response in excitatory but not inhibitory synapses of Mint deficient neurons. Data shown represent absolute mean EPSP and IPSP amplitudes. E, F, Plots of 10–90% rise times and half-width showed no changes. G, H, Traces of synaptic depression responses recorded from excitatory and inhibitory neurons, respectively, during a 10 Hz stimulation.