Figure 7.
Delay fear conditioning. Freezing responses during training (a-c), 24 h context test freezing responses (d-f), and 24 h tone test freezing responses (g-i) are shown for pubertal males (left column), adult males (middle column), and adult females (right column). Control males are in solid lines with filled diamonds or in black bars, Wa-1 males are in dotted lines with open diamonds or in gray bars, control females are in solid lines with filled circles or in white bars, and Wa-1 females are in dotted lines with open circles or in diagonally patterned bars. Only Wa-1 adult males show a deficit in the CR2, context test, pre-tone, and tone tests. The tone learning is significantly reduced in adult male Wa-1, even after normalized to the reduction in pre-tone freezing. The asterisks indicate statistically significant (mean ± SEM; p < 0.05) differences between control and mutants. T, Tone-shock paired trial; CR, conditioned response.