Figure 7.
Hsp104 does not protect against 6-OHDA-induced neurodegeneration. A, Comparison of the protective effects observed between torsins and yeast Hsp104 overexpressed in DA neurons. After 6-OHDA treatment, worms were examined for the presence of all four wild-type CEP neurons at specific time points. Filled circle, TOR-2; open circle, torsinA; open square, Hsp104; filled square, N2 background with only Pdat-1::GFP. Statistical significance between Pdat-1::GFP and each of the other transgenic lines was assessed by the ANOVA Bonferroni's test (*p < 0.05; NS, not significant). Error bars indicate SEM between independent experiments. B, Western analysis of Hsp104 expression in which Hsp104 was identified as a ∼100 kDa band present in extracts from Pdat-1::Hsp104 animals but not from N2 controls. Actin was probed as a loading control.