Figure 3.
Absence of HIF-1α is protective during global ischemia. A, A representation of acid fusin- and cresyl violet-stained sections from HIF-1αF/F (wild-type) and HIF-1αΔ/Δ (mutant) hippocampus shows a substantial decrease in neuronal damage in the HIF-1αΔ/Δ mice. Arrows indicate regions of neuronal damage. B, Mean neuronal damage in the cortex and hippocampus (Hipp) in HIF-1αF/F and HIF-1αΔ/Δ mice after a 75 min BCCAo and 3 d reperfusion is shown. Ischemic neuronal damage was graded on a four-point scale basis. Neuronal damage in the cortex and hippocampus was significantly greater in HIF-1αF/F (n = 6) versus HIF-1αΔ/Δ (n = 13) mice. *p < 0.05; **p < 0.005. Sections were viewed at 2× magnification. The data were compared using a Student's t test.