Figure 6.
Subunit-specific surface delivery of NMDA receptors. A, Representative traces of surface-delivered NMDAR-mediated currents with (dotted line) or without (thick line) ifenprodil after 2 d of TTX treatment. The neurons were pretreated with both MK-801 and NMDA to block all NMDARs expressed at the neuronal surface. B, Total NMDA currents in each control (Ctrl) and TTX-treated (TTX) neurons were measured before and after the addition of ifenprodil. Averaged data are indicated in bars (Ctrl, 2.88 ± 0.37 × 10-10 C, n = 14; Ctrl + ifenprodil, 2.24 ± 0.31 × 10-10 C, n = 14; TTX, 4.77 ± 0.30 × 10-10 C, n = 11; TTX + ifenprodil, 1.78 ± 0.22 × 10-10 C, n = 11). C, Subunit typing of surface-delivered NMDARs. NR2B-NMDAR- and non-NR2B-NMDAR-mediated currents were determined in control and TTX-treated neurons (Ctrl, n = 9; TTX, n = 23; *p < 0.05).