Skip to main content
. 2005 May 4;25(18):4659–4671. doi: 10.1523/JNEUROSCI.0565-05.2005

Figure 1.

Figure 1.

Nissl staining of NCAM-EC transgenic brain and immunocytochemical localization of NCAM-EC protein. A, B, Nissl staining of adult WT and NCAM-EC brain in a coronal section through the hippocampus (HC), thalamus (th), corpus callosum (cc), lateral ventricle (LV), and dorsal third ventricle (D3V). Scale bar, 500 μm. Immunoperoxidase staining with anti-HA antibodies in adult transgenic cingulate cortex (C) shows localization in layers I-VI and no staining in WT cortex (D). Scale bar, 100 μm. E, NCAM-EC immunoreactivity in the cingulate of transgenic mice is evident in the neuropil and soma of large pyramidal-like neurons (arrowhead) and nonpyramidal-like cells (arrow). Scale bar, 50 μm. F, NCAM-EC staining in the hippocampus of transgenic mice in CA1, CA2, and CA3 and the dentate gyrus (DG). G, No staining in WT hippocampus. Scale bar, 100 μm. H, NCAM-EC staining in the hippocampus and dentate gyrus is evident in the neuropil and cell bodies. Scale bar, 150 μm. I, NCAM-EC staining in the basolateral nucleus of amygdala of transgenic mice. J, No staining in WT amygdala. Scale bar, 100 μm. K, NCAM-EC staining in the cerebellum of transgenic mice. Scale bar, 100 μm. IGL, Internal granular layer; Mo, molecular layer.