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Colocalization of NR1/GluR2/3 (A-F) and NR1/synaptophysin (G-I) immunostaining in 9-d-old cultures. Each row of three images shows double labeling of a different field. Images of immunostained NR1 (A, D, G), GluR2/3 (B, E), and synaptophysin (H) clusters were combined (C, F, I) to show that some of the NR1-containing clusters are colocalized with GluR2/3-containing (C, F) or synaptophysin-containing (I) clusters (long arrows), whereas others are not (short arrows). Scale bar, 2 μm.
