Figure 5.
Glycine increases both total surface GluR1 expression and synaptic GluR1 expression in PFC neurons. A, Quantification of the effect of glycine on total cell-surface GluR1 staining. Both 10 and 100 μm glycine significantly increased surface GluR1 expression in PFC neurons compared with their respective control groups (t tests; *p < 0.05), whereas 1 μm glycine did not change GluR1 surface expression (t test; p > 0.05). Results are presented as the mean area of GluR1 puncta, normalized to the control group. B, Quantification of the effect of glycine on synaptic expression of GluR1. Both 10 and 100 μm glycine significantly increased synaptic GluR1 expression in PFC neurons compared with their control groups (t tests; *p < 0.05), whereas 1 μm glycine did not change synaptic GluR1 expression (t test; p > 0.05). Results are presented as the percentage of total SB area that overlaps with GluR1 area, normalized to the control group. Error bars indicate SEM.