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Transfection of Shank3 induces de novo formation of dendritic spines in CGCs. A, Immunolabeling of endogenous Shank3 and PSD-95 in a CGC. Scale bars: 10 μm unless otherwise specified; inset, 2.5 μm. B, GFP-transfected CGC. Scale bars: 5 μm; inset, 3 μm. C, GFP fluorescence of a myc-Shank3-cotransfected CGC. Scale bar: inset, 5 μm. D, Typical GFP fluorescence and myc-Shank3 immunostaining of a dendritic spine from a GFP plus myc-Shank3-cotransfected CGC. Scale bar, 2.5 μm.
