Figure 2.
Block of high-threshold K+ currents has a frequency-dependent effect on the F-I relationship and spike waveform shape of ELL pyramidal cells. A, Bath-applied 500 μm TEA selectively reduces the gain response of the F-I relationship in the high-frequency region while having little effect at low frequencies. The difference in firing frequency between control and TEA conditions is subtracted and plotted as a function of the control frequency (middle panel). The average difference between the low-frequency (80-120 Hz) and high-frequency (200-240 Hz) firing rates is significantly different (right) between TEA and control conditions (*p < 0.01; n = 4). B, Bath-applied 500 μm TEA has a larger effect on general waveform shape at high frequencies while having little impact when the neuron is firing at low frequencies. The 10th spike in the spike trains is shown enlarged and superimposed (right panel).