Figure 2.
Proinflammatory cytokines inhibit fAβ-elicited phagocytosis by a transcriptionally dependent mechanism. A, BV-2 cells were incubated with IL-1β (15 ng/ml) for indicated time periods before fAβ25–35 (60 μm) stimulation for 30 min and the addition of fluorescent microspheres for 30 min. BV-2 cells were incubated with parthenolide (15μm)(B), SN50 (100μg/ml) (C), or SN50M (100 μg/ml) (C) for 3 h before stimulation with IL-1β (15 ng/ml) for 3 h. The cells were then incubated with fAβ25–35 (60μm) for 30 min, followed by the addition of fluorescent microspheres for 30 min. The fraction of phagocytically active cells was then determined. *p < 0.01 and **p < 0.001 compared with control. The data are representative of three independent experiments.