Figure 5.
Purkinje cell migration defects in src–/–fyn–/– cerebellum. Coronal sections of E18.5 cerebellum were stained with antibody to calbindin and 4′,6′-diamidino-2-phenylindole dihydrochloride (DAPI) to show nuclei. Each figure is a montage of high-power images. a, Purkinje cells in the wild-type cerebellum had largely migrated out to form the Purkinje cell layer. b, In the dab1–/– cerebellum, Purkinje cells were located in clusters deep in the cerebellum. c, A partial phenotype was seen in the src–/–fyn–/– cerebellum, where a vestigial partial Purkinje cell layer had formed, but most Purkinje cells were in clusters deep in the cerebellum. In all cases, an external granule layer could be detected. mb, midbrain; pcl, Purkinje cell layer; Pj, ectopic Purkinje cells; egl, external granule layer; vz, ventricular zone; v4, fourth ventricle. The asterisks indicate fiber tracts.