Figure 1.
Expression of VGLUT3 in medullary raphe neurons. a, Immunoperoxidase staining for VGLUT3 in medullary raphe regions. VGLUT3 immunoreactivity was localized in neuronal cell bodies as well as fibers and terminals. b, No immunoreactivity was observed after preincubation of the anti-VGLUT3 antibody with the antigenic peptide. c, In situ hybridization for VGLUT3 mRNA in medullary raphe regions. Hybridization signals for VGLUT3 mRNA were exhibited by medullary raphe neurons (arrows). d, Double immunofluorescence labeling for VGLUT3 (green) and serotonin (red) in medullary raphe regions. VGLUT3-immunoreactive neurons (filled arrowheads) and serotonin-immunoreactive neurons (open arrowheads) were clearly visualized. e, Brain maps showing the distribution of VGLUT3-immunoreactive neurons and serotonin-immunoreactive ones. Immunoreactive cell bodies in a 20-μm-thick frontal section of the corresponding rostrocaudal position were plotted on a drawing. DAO, Dorsal accessory olivary nucleus; MAO, medial accessory olivary nucleus; PIO, principal inferior olivary nucleus; py, pyramidal tract. Scale bars: a-c (in c), 50 μm; d, 30 μm; e, 500 μm.